Chonluten Research Information
Respiratory Support Tripeptide
EDG Tripeptide Bioregulator for Respiratory Research
Important: This information is for educational and research purposes only. Always consult with a qualified healthcare professional before use.
Contents
What is Chonluten?
Chonluten is a synthetic tripeptide bioregulator with the amino acid sequence Glu-Asp-Gly (EDG), also known as T-34. It is a short-chain peptide with a molecular weight of 289.29 Da (C₁₁H₁₇N₃O₇) and is part of the Khavinson peptide bioregulator family, initially isolated from bronchial epithelial tissues [citation:1][citation:5][citation:10].
Chonluten has emerged as a promising molecular tool for investigating respiratory mucosal function, inflammatory pathway regulation, and lung epithelial cell homeostasis in research models. Its tissue-specific regulatory activity on respiratory epithelial cells makes it a valuable compound for respiratory system research [citation:1][citation:6].
Mechanism of Action
Chonluten functions through multiple pathways to support respiratory research models [citation:1][citation:5][citation:12]:
DNA Interaction
Penetrates cell nuclei and binds to specific DNA sequences (CTG motifs) in promoter regions, modulating gene transcription [citation:1][citation:5]
STAT Pathway Modulation
Alters STAT1 and STAT3 phosphorylation balance in macrophages, shifting immune responses away from chronic inflammatory signaling [citation:1][citation:6]
TNF Tolerance Induction
Inhibits TNF production in monocytes exposed to LPS, promoting attenuation of inflammatory action [citation:1][citation:5][citation:10]
Chonluten modulates gene expression including c-Fos, HSP70, SOD, COX-2, and TNF-alpha through promoter binding and epigenetic mechanisms. It also reduces pro-inflammatory cytokine expression (IL-6, IL-17) and alters immune cell adhesion dynamics [citation:1][citation:2][citation:5].
Research Applications
Respiratory System
Chronic bronchitis models, bronchial mucosal function, COPD research, hypoxic stress adaptation [citation:1][citation:2][citation:4]
Inflammatory Pathways
Cytokine regulation (IL-6, TNF-alpha, IL-17), immune cell adhesion modulation [citation:1][citation:5][citation:10]
Gastrointestinal Mucosal Research
Gastric and intestinal mucosal function, peptic ulcer models, inflammatory bowel disease research [citation:1][citation:6]
Cellular Aging Studies
Mesenchymal stem cell lifespan extension, senescence mechanisms [citation:1][citation:6]
Clinical Studies
Key Research Findings [citation:1][citation:2][citation:5]:
- Oral administration of Chonluten combined with Bronchogen enhanced effectiveness of standard therapy in patients with bronchopulmonary pathology including COPD and chronic bronchitis with asthmatic component [citation:2]
- Stress-protective effect associated with regulation of c-Fos gene expression, HSP70 heat shock protein gene, antioxidant enzyme genes (SOD), COX-2, and TNF-alpha [citation:2][citation:12]
- Inhibited in vitro tumor necrosis factor (TNF) production by monocytes exposed to pro-inflammatory bacterial lipopolysaccharide (LPS) [citation:1][citation:5][citation:10]
- Identified as a potential therapeutic agent for respiratory pathology associated with viral infections based on bronchial epithelium protective and anti-inflammatory properties [citation:2]
- Reduction in immune cell adhesion to activated endothelial cells, suggesting anti-inflammatory mechanisms [citation:1][citation:5]
Dosing Guide
Reconstitute 20mg vial with 3.0 mL bacteriostatic water = 6.67 mg/mL concentration. 1 unit = 0.01 mL ≈ 66.67 mcg on a U-100 insulin syringe.
Step 1: Select Vial Size
Step 2: Select Dose
Typical daily range: 250 mcg - 4 mg (gradual titration over 8-16 weeks)
Vial Size
20mg Vial
Selected Dose
500 mcg
Doses per Vial
40 doses
Units on Syringe
7.5 units
Volume
0.075 mL
Protocol
Weeks 3-4
Recommended Schedule
Daily - Subcutaneous Injection
Do not change your dose unless advised by a qualified professional.
Standard Gradual Protocol (3 mL = 6.67 mg/mL)
Gradual titration over 8-16 weeks. For ≤10 unit doses, use 30- or 50-unit insulin syringes.
Vial Size
20mg vial with 3.0mL bac water (6.67 mg/mL)
| Week | Daily Dose | Units on Syringe | Volume (mL) |
|---|
Always use a new sterile needle and syringe for each injection.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe
- Inject slowly down the vial wall; avoid foaming
- Gently swirl/roll until dissolved (do not shake)
- Label and refrigerate at 2-8°C (35.6-46.4°F), protected from light
Safety & Precautions
Do not use if you:
- Are allergic to Chonluten or any components
- Are pregnant or breastfeeding
Storage Instructions:
- Lyophilized: Refrigerate at 4°C (39.2°F) short-term or freeze at -20°C (-4°F) long-term
- After reconstitution: Refrigerate at 2-8°C (35.6-46.4°F)
- Avoid freeze-thaw cycles after reconstitution
Warning: If you experience severe allergic reactions, difficulty breathing, or signs of anaphylaxis, seek immediate medical help.
References
- Avolio F, Martinotti S, Khavinson VK, et al. Peptides Regulating Proliferative Activity and Inflammatory Pathways in the Monocyte/Macrophage THP-1 Cell Line. Int J Mol Sci. 2022;23(7):3607. doi:10.3390/ijms23073607 [citation:1][citation:5][citation:10]
- Khavinson VK, Lin'kova NS, Tarnovskaya SI. Short Peptides Regulate Gene Expression. Bull Exp Biol Med. 2016;162(2):288-292. doi:10.1007/s10517-016-3596-7 [citation:1]
- Khavinson VKh, Fedoreyeva LI, Vanyushin BF. Site-specific binding of short peptides with DNA modulated eukaryotic endonuclease activity. Bull Exp Biol Med. 2011 May;151(1):66-70 [citation:1]
- Fedoreyeva LI, Smirnova TA, Kolomijtseva GY, et al. Interaction of short peptides with FITC-labeled wheat histones and their complexes with deoxyribooligonucleotides. Biochemistry (Mosc). 2013 Feb;78(2):166-75 [citation:1]
- Khavinson VKh, Lin'kova NS, Dudkov AV, et al. Peptidergic regulation of expression of genes encoding antioxidant and anti-inflammatory proteins. Bull Exp Biol Med. 2012;152(5):615-618. doi:10.1007/s10517-012-1590-2 [citation:1][citation:12]
- Khavinson VK, Linkova NS, Mironova ES, et al. Peptide Regulation of Gene Expression: A Systematic Review. Molecules. 2021;26(22):7053. doi:10.3390/molecules26227053 [citation:1][citation:12]
This information is for educational and research purposes only. Always consult with a qualified healthcare professional.
Last updated: August 2026